Credits
This is a 2-credit hour course.
Description
Explores techniques in DNA analysis such as: Advanced sampling techniques, Y-STR analysis, high throughput screening, Next Generation Sequencing (NGS), single nucleotide polymorphisms (SNPs), mitochondrial DNA (mtDNA), low copy number DNA (LCN), automation, and other new technologies relevant to forensic DNA analysis.
Upon completion of this course, the student will be able to:
- Understand how advances in DNA technology are relative and relate to the field of forensic science, specifically in DNA analysis.
- Demonstrate understanding of advanced screening techniques as they apply to forensic examination of biological evidence.
- Be able to analyze the strategies for challenging short tandem repeat samples to include approaches for Low Copy Number analysis and degraded DNA.
- Demonstrate knowledge of alternative PCR-based technologies such as Y-STR, Mitochondrial, X-STR, and single nucleotide polymorphisms. Explain their value to the field of forensic biology.
- Demonstrate knowledge of binary mixture deconvolution strategies to include Random Match Probability, Combined Probability of Inclusion, and Likelihood Ratio.
Prerequisites
Forensic Analysis of DNA 1 required, Forensics Analysis of DNA 2 is preferred but not required.
Topics
| Module | Topic |
|---|---|
| Module 1 | Introduction to Advances in DNA Technologies |
| Module 2 | STR Amplification Techniques |
| Module 3 | STR Separation, and Detection Techniques |
| Module 4 | Emerging Technologies and Automation Strategies |
| Module 5 | Strategies for Analysis of Degraded and Low Copy Number (LCN) DNA |
| Module 6 | Alternative PCR Technologies: Y and X Chromosome Analysis Techniques |
| Module 7 | Alternative PCR Techniques: Mitochondrial and Single Nucleotide Polymorphism Analysis Techniques |
| Module 8 | Binary and Probabilistic Methods for Mixture Deconvolution |
| Model 9 | Genetic Genealogy and Phenotypic Profiling Applications |
Required Text
Advanced Topics in Forensic DNA Typing: Methodology; Published 2012
Author: John Butler
ISBN: 978-0-12-374513-2
Library Access
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